| 1. | The recombinant vector pqe - 80l / dhfr / hpd - 3 was identified by restriction digestion while we also construct the recombinant control vector pqe80l / dhfr with the same strategy . 3 同时还将表达运载蛋白dhfr的基因单独连接于表达载体pqe - 80l ,得到对照质粒pqe - 80l dhfr 。 |
| 2. | So we present two methods : the mod method by introducing mismatched control vector and the ssp algorithm by partitioning subspaces to reduce the dimensions of adaptive process 为此,我们提出了两种方法:通过引入失配方向控制向量而得到的最优失配检测方法和通过子空间划分而减小自适应维数而得到的子空间投影方法。 |
| 3. | ( 2 ) the construction of the recombinant prorarylic expression vector pqe80l / dhfr / hpd - 3 and the control vector pqe80l / dhfr . an about 135 bps fragment could be seen after pqe80l / dhfr / hpd - 3 vector was digested by kpn i + hindiii Sds一队ge分析可见重组原核表达载体pqe一80l / dhf侧hpd一3表达的目的蛋白带和对照质粒pqe一sol / dhfr表达的蛋白带,大小分别约为31kd和26kd 。 |
| 4. | 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr 将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ,经酶切鉴定,得到重组原核表达载体pqe - 80l dhfr h d - 3 。 |
| 5. | Discovery could quickly trigger measures to control vector proliferation without harming the environment , to advise the public about self - protection , to provide vaccines ( when available ) for at - risk populations and to deliver prompt treatments 一旦发现,就会快速采取措施在不破坏环境的情况下控制带菌媒介的繁殖,建议公众加强自我保护,为有危险的人群提供疫苗(有可能的话)以及及时的医治。 |
| 6. | Optimal predictive control to damp oscillation for linear time - delay systems with external sinusoidal disturbances is considered in chapter 4 . a full - order state predictive observer for time - delay systems by using the control vector and the predictive output vector of the controlled plant is structured 利用构造预测模型得到的被控对象预测输出向量和系统的控制向量,设计一种全维状态预测观测器,并将该状态观测器用于时滞控制系统的最优前馈-反馈预测控制中。 |